The ImmunoSensation Cluster of Excellence places strong emphasis on the support of early careers and gender equality. Our four-layered Promotion Concept for graduate students, post docs, young investigators and group leaders will be supported by our special funding program and will provide exciting new career opportunities that will be further shaped through interactions with our local and international partners.

____________________________________________________________

 

PhD position in Molecular Immunology

The University of Bonn/University Hospital Bonn is offering a


PhD position in Molecular Immunology


starting in November 2022. The position is placed as a joined project in the laboratory of Prof. Eva Kiermaier at the LIMES Institute and the laboratory of Prof. Katrin Paeschke at the University Hospital in Bonn (UKB). The Institutes are part of the Faculty of Mathematical and Natural Sciences and the Faculty of Medicine at the University of Bonn.


We are seeking for a highly motivated PhD candidate to work at the interface of molecular Immunology and Genetics. The project focuses on deciphering the role of genomic lesions and downstream signaling pathways for leukocyte biology. The project combines cell based in vitro assays with advanced imaging techniques and state-of-the-art genetic tools to provide a complete picture of the molecular basis and signaling modules of DNA repair mechanisms required for regular immune cell function.


The applicant should hold a master degree in cell/molecular biology, genetics or immunology. We particularly look for applicants with a collaborative attitude towards research and who can combine high personal ambition and work ethics with eagerness to contribute to other projects.


Salary is paid according to German TV-L (E13 65%). The University of Bonn is an equal opportunities employer. Complete applications in English should include a CV, a brief statement of research experiences and interests and addresses of two referees.

 

Please submit your application as a single pdf file to Eva Kiermaier (eva.kiermaier(at)uni-bonn.de) or Katrin Paeschke (paeschke(at)uni-bonn.de) before 15th of August 2022.

3 PhD positions (m/w/d)

The Institute of Clinical Chemistry and Clinical Pharmacology (research group of Prof. Dr. Kathrin Leppek) at the Faculty of Medicine of the Rheinische Friedrich-Wilhelms-University Bonn invites applications for

3 PhD student positions (m/f/d) (65% TV-L E13, 3 years)

Project Title: Transcript-specific translation by the ribosome

The Institute of Clinical Chemistry and Clinical Pharmacology represents the two areas of Clinical Chemistry and Clinical Pharmacology in research and healthcare. The institute includes the Central Laboratory of the University Hospital, the Phase I unit for early clinical trials and 11 scientific working groups. The institute is member of the Cluster of Excellence ImmunoSensation, the Center for Integrated Oncology (CIO) Cologne Bonn and the German Center for Infection Research (DZIF). The research focus is in innate immunity, specifically immune recognition of nucleic acids and tumor immunology.

lab description

Our lab focuses on how regulation of gene expression is directly executed by the ribosome, particularly in the innate immune response. Our lab studies a fundamentally new mode of gene regulation by which ribosomal RNA (rRNA) regions exposed on the outer shell of the ribosome bind to selective transcripts to control mRNA- and species-specific translation. We combine innovative RNA biochemistry and modern RNA-based technology development with model systems ranging from yeast to macrophages. Our ultimate goal is to decipher how rRNA-directed specialized translation shapes gene expression to understand the role of the ribosome in innate immune responses. Bonn is a major centre of immunological research in Germany and our lab is a new, growing, exciting, creative and inclusive place to mentor the next generation of scientists.

project description

Regulation of gene expression, the decision which proteins to make from an identical genome, is essential to specify cell types and tissues. The ribosome, one of life’s most ancient molecular machines, has recently been revealed to be an active regulator of gene expression. Ribosomes are not all identical in composition and do not translate all mRNAs equally: ribosomes preferentially translate specific transcripts to diversify gene expression. It is poorly understood how ribosome components, proteins and rRNA, mediate such selectivity. The regulatory capacity of rRNA in translation has long remained unexplored. Ribosomes have dramatically increased in size across eukaryotic evolution, due in part to less conserved sequence insertions called rRNA expansion segments (ESs) that “expand” rRNA on the outer ribosome shell. rRNA ESs neither contribute to nor interfere with rRNA’s essential role in peptide-bond formation, so why do they exist? And what do they do?

In several projects, we want to understand how rRNA ESs directly bind to selective transcripts to control mRNA- and species-specific translation (Leppek et al., 2020; Leppek, Byeon et al., 2021), how ribosomes recognize, bind and translate selective mRNAs such that rRNA-mRNA recognition patterns diversify the proteome. Ribosomes have thereby evolved the ability to discriminate which proteins are made in a cell through selective translation via rRNA. We aim to identify and characterize the molecular mechanisms of such ribosome-directed translation. The eukaryotic ribosome contains a multitude of distinct, unexplored ESs that all have different sequence and structure. Thus, there are many open questions about what other potential functions rRNA ESs on ribosomes may have in gene regulation. In particular, we view ribosome-regulation through the lens of the innate immune response of macrophages. In the innate immune response customized translation may underlie dynamic and rapid cellular specification.

We also continue to advance tailored RNA-based technologies such as RNA aptamer-based affinity purification or our recent VELCRO-IP (variable expansion segment-ligand chimeric ribosome-IP) method, a ribosome purification and mRNA-interaction strategy that harnesses the interspecies evolutionary change in rRNA ES sequence to engineer chimeric ribosomes. Beyond, we strive to design and employ ribosome- and RNA-focused technologies to ask hard biological questions and with applications in basic science as well as in RNA therapeutics.

selected publications

·  Leppek K*, Byeon GW*, Kladwang W*, Wayment-Steele HK*, Kerr CH*, Xu AF**, Kim DS**, Topkar VV, Choe C, Rothschild D, Tiu GC, Wellington-Oguri R, Fujii K, Sharma E, Watkins AM, Nicol JJ, Romano J, Tunguz B, Diaz F, Cai H, Guo P, Wu J, Meng F, Shi S, Eterna Participants, Dormitzer PR, Solórzano A, Barna M‡, Das R‡. Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics. Nature Comm. 2022 Mar;13(1): 1536,1-22.

·  Leppek K*, Byeon GW*, Fujii K, Barna M. VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide. Cell Reports, 2021 Jan 19;34(3):108629.

·  Leppek K, Fujii K, Quade N, Susanto TT, Boehringer D, Lenarčič T, Xue S, Genuth NR, Ban, N, Barna M. Gene- and species-specific Hox mRNA translation by ribosome expansion segments. Mol. Cell, 2020 Dec 17;80(6):980-995.e13.

·  Leppek K, Das R, Barna M. Functional 5’ UTR mRNA structures in eukaryotic translation regulation and how to find them. Nat Rev Mol Cell Biol. 2018 Mar;19(3):158-174.

·  Leppek K and Stoecklin G. An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins. Nucl Acids Res. 2014 Jan;42(2):e13.

·  Leppek K, Schott J, Reitter S, Poetz F, Hammond MC, Stoecklin G. Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs. Cell. 2013 May 9;153(4):869-81.

methods that will be used

·       Molecular Biology/ Biochemistry

·       In vitro cell culture/ mouse embryonic stem cells/ primary immune cells

·       Ribosome profiling/ Polysome profiling/ mRNA translation techniques

·       Yeast genetics/ engineering

·       CRISPR-based genome editing

·       RNA technology development

·       RNA structure-function assays/ RNA structure probing

·       RNA sequencing/ data analysis

·       FACS/ cell sorting

·       Mouse genetics (eventually/ in collaboration)

profile of cadidate's qualification

The candidate (m/f/d) should hold a M.Sc. degree (or equivalent) and be an enthusiastic and highly motivated student who can work independently in a team, and values creativity, innovation and collaboration. The ideal candidate should be excited to join an international research group in a highly dynamic working environment (with English as the main written/spoken language) and should be reliable, responsible and have high scientific standards. The candidate should have a biomedicine, RNA biology, immunology, biology or a biochemical and molecular biology background. Experience in data science and RNA-seq analysis is a plus and willingness to learn computational biology is ideal.

we offer

·       The salary will be according to the German salary scale TV-L

·       A “Jobticket” (subsidized public transport) is available

·       There is also a possibility to use the day care center

·       Supplementary benefits in the public sector (pension plan according to VBL)

 

The University of Bonn is committed to diversity and equal opportunity. It is certified as a family-friendly university. It aims to increase the proportion of women in areas where women are under-represented and to promote their careers in particular. It therefore urges women with relevant qualifications to apply. Applications will be handled in accordance with the Landesgleichstellungsgesetz (State Equality Act). Applications from suitable individuals with a certified serious disability and those of equal status are particularly welcome.

application / contact

For further details about the application procedure and inquiries, please send an email with your CV, letter of motivation and two contacts of reference in one single pdf file addressed to Prof. Dr. Kathrin Leppek preferably by email as soon as possible and before 31st August 2022 to:

Prof. Dr. Kathrin Leppek

TT W1 Jun.Prof., PhD (Dr. rer. nat.) / Immunobiochemistry Lab

Institute for Clinical Chemistry & Clinical Pharmacology

University of Bonn / University Clinic Bonn – Faculty of Medicine

Venusberg-Campus 1, Geb. B12, Biomedical Center II (BMZ II), Raum EG-412, 53127 Bonn, Germany

Phone (Office): +49 228 287-51158 / Phone (Lab): +49 228 287-51172 / Fax: +49 228 287-16094

email: kleppek(at)uni-bonn.de / kathrin.leppek(at)ukbonn.de

www.ukbonn.de/icccp/research/leppek-group / www.ukbonn.de / www.ukbonn.de/ikckp

Twitter: @KatLeppek / LinkedIn: www.linkedin.com/in/kathrin-leppek-1b3450a1/

Student Assistant or research Assistant (m/f/d)

The Department of Immunology and Metabolism at the Life and Medical Science (LIMES) Institute of the University of Bonn offers a position for

 

a Student Assistant or Research Assistant

(19 h /week for 6 months)

 

to support the research work of the department. Our working group is interested in the epigenetic and metabolic regulation of the immune cell responses. The LIMES Institute is an interdisciplinary scientific institution within the Faculty of Mathematics and Natural Sciences. As an interface between biology, biochemistry and the medical disciplines, it also has a bridging function to other faculties and the public. Further information can be found on the institute's website: www.limes-institut-bonn.de.

 

 

Type of work

  • Sample preparation: isolation of immune cells from mouse tissues
  • Flow cytometry staining, cell cultures
  • Evaluation and data analysis

The working hours are based on the requirements of the laboratory, but can also be arranged flexibly within this framework. The salary is based on the remuneration rate of the University of Bonn for student and research assistants.

Please send applications with a short cover letter and CV as a single PDF file by June 15th to Dr. Katarzyna Placek: kplacek@uni-bonn.de

 

 

Katarzyna Placek, PhD
University of Bonn
Life & Medical Science Institute (LIMES)
Dpt. Immunology and Metabolism
Carl-Troll-Straße 31
53115 Bonn

0228 / 73 62789

PhD Position in Neurovascular Cell Biology (m/f/d)

The central nervous system (CNS) (brain and spinal cord) is composed of
different neural cell types organized in a very stereotyped pattern and
localized in defined compartments. Apart of the neuronal cell types, the
CNS is also composed of blood vessels, responsible for providing oxygen
and nutrients to the neuronal tissue. Similar to neurons, during
development, blood vessels also follow defined pathways to enter and
invade the CNS in order to assure proper oxygen and nutrient delivery to
all parts of the tissue.


In this project we aim to identify and characterize the molecular
mechanisms of the bidirectional communication between neural cells and
cells forming the vasculature in development, homeostasis and disease. In
disease, the interaction of the vasculature with the immune system will also
be explored. We intend to understand how different neuronal populations
control CNS vascularization and how the vasculature also actively
contributes to the development and maintrenance of CNS homeostasis.


References


Luck R, Karakatsani A, Shah B, Schermann G, Adler H, Kupke J, Tisch N, Jeong HW, Back MK, Hetsch F, D`Errico A, De Palma M, Wiedtke E, Grimm D, Acker-Palmer A, von Engelhardt J, Adams RH, Augustin HG, Ruiz de Almodovar C. The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner. Cell Rep. 2021, 36(7), 109522.

Paredes I, Vieira JR, Shah B, Ramunno CF, Dyckow J, Adler H, Richter M, Schermann G, Giannakouri E, Schirmer L, Augustin HG, Ruiz de Almodovar C. Oligodendrocyte precursor cell specification is regulated by bi-directional neural progenitor-endothelial cell crosstalk. Nat Neurosci. 2021, 24, 478–488.

Vieira JR, Shah B, Ruiz de Almodovar C. Cellular and molecular mechanisms of spinal cord Institute for Neurovascular Cell Biologyvascularization. Front Physiol 2020. Dec 21

Luck R*, Urban S*, Karakatsani A, Harde E, Sambandan S, Nicholson L, Haverkamp S, Mann R, Martin-Villalba A, Schuman EM, Acker-Palmer A, Ruiz de Almodóvar C. VEGF/VEGFR2 signaling regulates hippocampal axon branching during development. Elife. 2019 Dec 23;8.

Karakatsani A, Shah B, Ruiz de Almodovar C. Blood vessels as regulators of neural stem cell properties. Front Mol Neurosci 2019 Apr 12;12:85

Paredes I, Himmels P, Ruiz de Almodovar C. Neuro-vascular communication in the CNS during development. Dev Cell. 2018. Apr 9;45(1):10-32

Wang X, Freire Valls A, Shermann G, Shen X, Moya IM, Castro L, Urban S, Solecki GM, Winkler F, Riedemann Lm Jain RK, Mazzone M, Schmidt T, Fischer T, Halder G, Ruiz de Almodovar C. YAP/TAZ orchestrate VEGF signaling during developmental angiogenesis. Dev Cell. 2017 Sep 11;42(5):462-478.e7.

Himmels P, Paredes I, Karakatsani A, Luck R, Marti H, Rempel E. Stoeckli E & Ruiz de Almodovar, C. Motoneurons guide blood vessels in the developing spinal cord via a sFlt1 dependent mechanism. Nat Commun. 2017 Mar 6;8:14583.


Methods that will be used


Single cell sequencing and spatial transcriptomics
Mouse genetics
Micro-dissections
Ex vivo 3D cell culture
In vitro cell culture
Cell Biology
Biochemistry and Molecular Biology techniques
Histology
Immunohistochemisty
Light and fluorescent microscopy
Confocal microscopy


Profile of candidate’s qualification

The candidate should be an enthusiastic and motivated student willing tojoin an international research group in a highly dynamic working environment (with English as main language). The ideal candidate should have a biomedicine, neuroscience, biology or a biochemical and molecular biology background.


Application

For further details on the application procedure, please
send an email with your CV, letter of motivation and contacts for
references to: carmen.ruizdealmodovar(at)ukbonn.de

 

Prof. Dr. Carmen Ruiz de Almodóvar
Director
Schlegel Chair
Tel. +49 228 688-530328
carmen.ruizdealmodovar@ukbonn.de
Life & Brain Center
University hospital Bonn
Venusberg-Campus 1, Geb. 76
53127 Bonn

BTA / CTA (m/w/d)

Das Universitätsklinikum Bonn (UKB) ist ein Krankenhaus der Maximalversorgung mit mehr als 1.306 Planbetten. Unsere mehr als 8.000 Mitarbeiterinnen und Mitarbeiter übernehmen Aufgaben in Forschung, Lehre und Krankenversorgung sowie im öffentlichen Gesundheitswesen auf höchstem Niveau. Das UKB hat in 2021 den vierthöchsten Case-Mix-Index sowie das beste wirtschaftliche Ergebnis der Universitätskliniken in Deutschland und steht im Wissenschaftsranking (LOMV) auf Platz 1 der Universitätsklinika in NRW.

In der Arbeitsgruppe Prof. Florian Schmidt im Institut für angeborene Immunität (Direktor: Prof. Dr. Felix Meissner, Prof. Dr. Eicke Latz, Prof. Dr. Dagmar Wachten) des Universitätsklinikums Bonn ist zum 01.10.2022 oder ab sofort folgende Stelle in Vollzeit (38,5 Std./Woche) zu besetzen:

 

Biologisch Technischer Assistent / Chemisch Technischer Assistent (m/w/d)

 

Die Stelle ist aufgrund des Projekts „Elucidating inflammasome activation with alpaca single domain antibodies (VHHs)“ befristet für ein Jahr zu besetzen.

 

Ihre Aufgaben

  • Molekularbiologie (PCR, Gelelektrophorese, RNA- und DNA Extraktion, Bakterienkultur, Klonieren)
  • Identifizierung von Alpaka Antikörpern (Nanobodies) per Phage Display
  • Expression und Aufreinigung von Alpaka Nanobodies
  • Organisation von Alpaka-Immunisierungen und Nanobody-Libraries
  • Zellkultur

 

Ihr Profil

  • Abgeschlossene Ausbildung als MTLA, BTA, oder CTA oder gleichwertige Qualifikationen (m/w/d)
  • Praktische Erfahrungen in der Zellkultur, Molekularbiologie und Proteinbiochemie
  • Eigenverantwortliches Arbeiten, hohe Einsatzbereitschaft
  • Allgemeine Computer-Kenntnisse und fundierte Grundlagen in Microsoft Excel
  • Sehr gute Englischkenntnisse (im Institut wird in Englisch kommuniziert)
  • Teamorientiert und engagiert
  • Motiviert und begeistert an Forschungstätigkeiten in einem internationalen Team
  • Verlässlich und fähig eigenständig zu arbeiten
  • Flexibel (kurze Arbeitsschritte können samstags und an Feiertagen notwendig sein)

 

Wir bieten

  • Verantwortungsvoll und vielseitig: ein Arbeitsplatz mit großem Gestaltungsspielraum in einem kollegialen Team
  • Sicher in der Zukunft: Entgelt nach TV-L
  • Flexibel für Familien: flexible Arbeitszeitmodelle, Möglichkeit auf einen Platz in der Betriebskindertagesstätte und Angebote für Elternzeitrückkehrer
  • Vorsorgen für später: Betriebliche Altersvorsorge
  • Clever zur Arbeit: Großkundenticket des öffentlichen Nahverkehrs VRS oder Möglichkeit eines zinslosen Darlehens zur Anschaffung eines E-Bikes
  • Bildung nach Maß: Geförderte Fort- und Weiterbildung
  • Start mit System: Strukturierte Einarbeitung
  • Gesund am Arbeitsplatz: Zahlreiche Angebote der Gesundheitsförderung
  • Arbeitgeberleistungen: Vergünstigte Angebot für Mitarbeiter*innen

Wir setzen uns für Diversität und Chancengleichheit ein. Unser Ziel ist es, den Anteil von Frauen in Bereichen, in denen Frauen unterrepräsentiert sind, zu erhöhen und deren Karrieren besonders zu fördern. Wir fordern deshalb einschlägig qualifizierte Frauen nachdrücklich zur Bewerbung auf. Bewerbungen werden in Übereinstimmung mit dem Landesgleichstellungsgesetz

behandelt. Die Bewerbung geeigneter Menschen mit nachgewiesener Schwerbehinderung und diesen gleichgestellten Personen ist besonders willkommen.

 

Kontakt

Sie erfüllen unsere Anforderungen und suchen eine abwechslungsreiche und herausfordernde Tätigkeit? Zögern Sie nicht und senden Sie Ihre aussagekräftige Bewerbung (bevorzugt per E-Mail in einer Datei bis 5 MB Größe) bis zum 09.08.2022 unter Angabe der Stellenanzeigen-Nr. 634_2021 an:

 

Dr. Florian Schmidt

Institut für Angeborene Immunität (BMZ)

Universitätsklinikum Bonn

Venusberg-Campus 1, 53127 Bonn

Tel.: 0228/287-51239

E-mail:

fschmidt(at)uni-bonn.de

leonie.verwohlt(at)ukbonn.de

www.ukbonn.de