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ACS Chem Neurosci. 2019 Jul 11.

Blocking inflammasome activation caused by β-amyloid peptide (Aβ) and islet amyloid polypeptide (IAPP) through an IAPP mimic.

Aftabizadeh M, Tatarek-Nossol M, Andreetto E, El Bounkari O, Kipp M, Beyer C, Latz E, Bernhagen J, Kapurniotu A

Inflammation in brain and pancreas is linked to cell degeneration and pathogenesis of both Alzheimer's-disease (AD) and type 2-diabetes. Inflammatory cascades in both tissues are triggered by the uptake of β-amyloid-peptide (Aβ) or islet-amyloid-polypeptide (IAPP) aggregates by microglial-cells (AD) or macrophages (T2D) and their insufficient lysosomal degradation. This results in lysosomal damage, caspase-1/NLRP3-inflammasome activation and release of interleukin-1β (IL-1β), a key proinflammatory cytokine in both diseases. Here we show that the inflammatory processes mediated by Aβ and IAPP aggregates in microglial cells and macrophages are blocked by IAPP-GI, a non-amyloidogenic IAPP mimic, which forms high-affinity soluble and non-fibrillar hetero-oligomers with both polypeptides. In contrast to fibrillar Aβ aggregates, non-fibrillar Aβ/IAPP-GI or Aβ/IAPP hetero-oligomers become rapidly internalized by microglial cells and targeted to lysosomes where Aβ is fully degraded. Internalization occurs via IAPP receptor-mediated endocytosis. Moreover, in contrast to IAPP aggregates, IAPP/IAPP-GI hetero-oligomers become rapidly internalized and degraded in the lysosomal compartments of macrophages. Our findings uncover a previously unknown function for the IAPP/Aβ cross-amyloid interaction and suggest that conversion of Aβ or IAPP into lysosome-targeted and easily degradable hetero-oligomers by hetero-association with IAPP mimics could become a promising approach to specifically prevent amyloid-mediated inflammation in AD, T2D or both diseases.

PMID: 31295403