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Neuropsychopharmacol Rep . 2020 Oct 10

Forskolin rapidly enhances neuron-like morphological change of directly induced-neuronal cells from neurofibromatosis type 1 patients

Marco A Ataide, Karl Komander, Konrad Knöpper, Annika E Peters, Hao Wu, Sarah Eickhoff, Tea Gogishvili, Justus Weber, Anika Grafen, Axel Kallies, Natalio Garbi, Hermann Einsele, Michael Hudecek, Georg Gasteiger, Michael Hölzel, Martin Vaeth, Wolfgang Kastenmüller, Jishnu Das, Anush Devadhasan, Caitlyn Linde, Tom Broge, Jessica Sassic, Max Mangano, Sean O'Keefe, Todd Suscovich, Hendrik Streeck, Alivelu Irrinki, Chris Pohlmeyer, Gundula Min-Oo, Shu Lin, Joshua A Weiner, Thomas Cihlar, Margaret E Ackerman, Boris Julg, Steven Deeks, Douglas A Lauffenburger, Galit Alter, Georg Nickenig, Marcel Weber, Robert Schüler, Jörg Hausleiter, Michael Nabauer, Ralph Stephan von Bardeleben, Efthymios Sotiriou, Ulrich Schäfer, Florian Deuschl, Hannes Alessandrini, Felix Kreidel, Jean-Michel Juliard, Eric Brochet, Azeem Latib, Matteo Montorfano, Eustachio Agricola, Stephan Baldus, Kai P Friedrichs, Shekhar H Deo, Suzanne Y Gilmore, Ted Feldman, Rebecca T Hahn, Francesco Maisano, Aline H de Nooijer, Inge Grondman, Nico A F Janssen, Mihai G Netea, Loek Willems, Frank L van de Veerdonk, Evangelos J Giamarellos-Bourboulis, Erik J M Toonen, Leo A B Joosten, Noriaki Sagata, Shin-Ichi Kano, Masahiro Ohgidani, Shogo Inamine, Yasunari Sakai, Hiroki Kato, Keiji Masuda, Takeshi Nakahara, Makiko Nakahara-Kido, Shouichi Ohga, Masutaka Furue, Akira Sawa, Shigenobu Kanba, Takahiro A Kato

Aim: Neurofibromatosis type 1 (NF1) is a multifaceted disease, and frequently comorbid with neurodevelopmental disorders such as autism spectrum disorder (ASD) and learning disorder. Dysfunction of adenylyl cyclase (AC) is one of the candidate pathways in abnormal development of neuronal cells in the brain of NF1 patients, while its dynamic abnormalities have not been observed. Direct conversion technology can generate induced-neuronal (iN) cells directly from human fibroblasts within 2 weeks. Just recently, we have revealed that forskolin, an AC activator, rescues the gene expression pattern of iN cells derived from NF1 patients (NF1-iN cells). In this microreport, we show the dynamic effect of forskolin on NF1-iN cells.

PMID: 33037790