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Int Immunol . 2020 Oct 29

Psoriasis-like skin disorder in transgenic mice expressing a RIG-I Singleton-Merten syndrome variant

Ahmed Abu Tayeh, Masahide Funabiki, Shota Shimizu, Saya Satoh, Lee Sumin, Yoichiro Iwakura, Hiroki Kato, Takashi Fujita

Mutations in DDX58, which encodes the cytoplasmic RNA sensor RIG-I, were recently identified in the rare autoimmune disease Singleton-Merten syndrome (SMS). We report the spontaneous development of psoriasis-like skin lesions as an SMS-like symptom in transgenic mice harboring one of the RIG-I SMS variants, E373A. Histological analysis revealed typical characteristics of psoriasis, including the abnormal proliferation and differentiation of keratinocytes leading to epidermal hyperplasia, and infiltrates consisting of neutrophils, dendritic cells and T cells. Levels of the IL-23/IL-17 immune axis cytokines were high in the skin lesions. Rag2-/- transgenic mice showed partial amelioration of the phenotype, with downregulation of inflammatory cytokines, including IL-17A, suggesting the importance of lymphocytes for the pathogenesis similar to that of human psoriasis. Of note, IL-17A deficiency abolished the skin phenotype and treatment using the JAK inhibitor tofacitinib not only prevented onset, but also improved the skin manifestations even after onset. Our study provides further evidence for the involvement of RIG-I activation in the onset and progression of psoriasis via type I interferon signaling and the IL-23/IL-17 axis.

PMID: 33119735