Prof. Dr. med. Joachim L. Schultze
Life & Medical Sciences Institute (LIMES)
j.schultze@uni-bonn.de View member: Prof. Dr. med. Joachim L. Schultze
Methods in molecular biology (Clifton, N.J.)
Laser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples.
© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PMID: 41629717