MetRS*-based deep cell-selective tissue proteomics and secretomics in vivo.

Combining MetRS*-based cell-selective protein labeling with mass spectrometry-based proteomics is a powerful approach for investigating intercellular communication within tissues. Cell-selective labeling overcomes limitations of cell sorting techniques and facilitates cell type-specific proteome and secretome analyses in vivo. Our recent work has showcased the application of this method for the comprehensive proteomic characterization of cellular proteins in tissues, as well as released proteins in the bloodstream. Here, we present experimental guidelines for MetRS*-based cell-selective proteomics experiments in vivo and a detailed sample preparation protocol for tissues and body fluids.

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PMID: 41276344