Skip to main content

Efficient Expression and Purification of Recombinant Mouse Dimeric IgA.

European journal of immunology

Authors: Antonia Geisse, Tao Zhang, Jonathan Schreiber, Kristina Markova, Sophie Burkhalter, Hedda Wardemann, Andrew J Macpherson, Tim Rollenske

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

© 2025 The Author(s). European Journal of Immunology published by Wiley‐VCH GmbH.

PMID: 40952320

Participating cluster members