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Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging.

Methods in molecular biology (Clifton, N.J.)

Authors: David Alejandro Bejarano, Andreas Schlitzer

Macrophages display a high degree of phenotypic diversity and plasticity, which is influenced by their location within the tissue microenvironment. Co-Detection by Indexing (CODEX), a multiplexed imaging technique, allows the simultaneous detection of multiple membrane and cellular markers that enable the accurate identification of tissue-resident hematopoietic and non-hematopoietic cells, while conferring spatial information at a single-cell level. Here we describe the use of CODEX to visualize the phenotypic and spatial heterogeneity of murine tissue-resident macrophages in several organs, and a pipeline to characterize their cellular microenvironments and interactions.

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PMID: 37639130

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